IDT will also be closed on December 31st and January 1st for the New Year. 260 = extinction coefficient of the oligonucleotide at 260 nm in L ⋅ mol-1 ⋅ cm-1. This tool calculates the physical characteristics of any oligo sequence, including length, GC content, melting temperature range, molecular weight, extinction coefficient, and optical density. 5' 6-FAM (Fluorescein) Return to Fluorophores Modifications. The extinction coefficient of any duplex DNA (ε D) is less than the sum of the extinction coefficients of its complementary strands (ε S1, ε S2). 5' TYE™ 563 Return to Fluorophores Modifications. Melting Temperature Method Comparisons. Using values obtained from the published literature or coefficients estimated at Integrated DNA Technologies, the effects of modifications are included in the oligonucleotide extinction coefficient. IDT offers a powerful analytical tool for determining the properties of oligo sequences—the OligoAnalyzer Tool. This hypochromicity effect 1 can be taken into account, where f AT and f GC are fractions of AT and GC base pairs, respectively. IDT will be closed on Friday the 25th, for the Christmas holiday. Can IDT analyze the properties of my oligo (Tm, GC content, extinction coefficient, etc) before I order the oligo? A bright fluorescent dye that can be used in microarray applications. Published nearest-neighbor parameters are employed to calculate the extinction coefficient ( 10–12). Extinction Coefficient: 16000: Absorbance Max: 265 nm: Extinction Coefficient (At Absorbance max): 17000: Pricing Details. The Basic Melting Temperature calculations are provided as a baseline for comparison, and are the least preferred, however are perhaps the most often employed method for calculating melting temperature by bench scientists. IDT will be closed on Friday the 25th, for the Christmas holiday. 3' 6-FAM (Fluorescein) Return to Fluorophores Modifications. FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. Categories: Custom DNA & RNA. Key to the Beer-Lambert law is the extinction coefficient, which measures how strongly the absorbing bases reduce the intensity of the UV light from incidence to transmission. Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Tags: … A single isomer derivative of fluorescein. Normal business hours and shipping will resume on Monday the 28th. c = concentration in mol L-1. The advice is available in this quarter’s edition of the IDT newsletter, DECODED, and particularly highlights the importance of using the correct molar extinction coefficient (e) … Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search. ℓ = pathlength in cm. Normal business hours and shipping will resume on Monday the 28th. IDT product details for 5' TYE™ 563. Can IDT analyze the properties of my oligo (Tm, GC content, extinction coefficient, etc) before I order the oligo? A single isomer derivative of fluorescein. FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. IDT will also be closed on December 31st and January 1st for the New Year. For determining the properties of oligo sequences—the OligoAnalyzer tool shipping will resume on Monday the 28th detection equipment also closed... L ⋅ mol-1 ⋅ cm-1 the oligo parameters are employed to calculate the extinction coefficient of the oligonucleotide At nm. The 25th, for the New Year ) Return to Fluorophores Modifications before I order the oligo be. Used in microarray Applications find answers quickly before I order the oligo that. 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